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Correlation between the BACTEC MGIT 960 culture system with Genotype MTBDRplus and TB-SPRINT in multidrug resistant Mycobacterium tuberculosis clinical isolates from Brazil.
Dantas, Nayanne Gama Teixeira; Suffys, Phillip Noel; Carvalho, Wânia da Silva; Gomes, Harrison Magdinier; Almeida, Isabela Neves de; Figueiredo, Lida Jouca de Assis; Gonçalves, Alan Douglas; Gomgnimbou, Michel Kireopori; Refregier, Guislaine; Sola, Christophe; Miranda, Silvana Spíndola de.
Afiliación
  • Dantas NGT; Universidade Federal de Minas Gerais, Faculdade de Medicina, Departamento de Clínica Médica, Programa de Pós-Graduação em Infectologia e Medicina Tropical, Belo Horizonte, MG, Brasil.
  • Suffys PN; Fundação Oswaldo Cruz-Fiocruz, Instituto Oswaldo Cruz, Laboratório de Biologia Molecular Aplicada a Micobactéria, Rio de Janeiro, RJ, Brasil.
  • Carvalho WDS; Universidade Federal de Minas Gerais, Faculdade de Farmácia, Departamento de Farmácia Social, Laboratório de Biologia Molecular e Saúde Pública, Belo Horizonte, MG, Brasil.
  • Gomes HM; Fundação Oswaldo Cruz-Fiocruz, Instituto Oswaldo Cruz, Laboratório de Biologia Molecular Aplicada a Micobactéria, Rio de Janeiro, RJ, Brasil.
  • Almeida IN; Universidade Federal de Minas Gerais, Faculdade de Medicina, Departamento de Clínica Médica, Programa de Pós-Graduação em Infectologia e Medicina Tropical, Belo Horizonte, MG, Brasil.
  • Figueiredo LJA; Universidade Federal de Minas Gerais, Faculdade de Farmácia, Departamento de Farmácia Social, Laboratório de Biologia Molecular e Saúde Pública, Belo Horizonte, MG, Brasil.
  • Gonçalves AD; Fundação Ezequiel Dias, Belo Horizonte, MG, Brasil.
  • Gomgnimbou MK; Centre Muraz, Bobo-Dioulasso, Burkina Faso.
  • Refregier G; Institut for Integrative Cell Biology, UMR9198 CEA-CNRS-UPSaclay, Orsay, France.
  • Sola C; University Paris-Sud, Beamedex SAS, Orsay, France.
  • Miranda SS; Institut for Integrative Cell Biology, UMR9198 CEA-CNRS-UPSaclay, Orsay, France.
Mem Inst Oswaldo Cruz ; 112(11): 769-774, 2017 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-29091137
ABSTRACT

BACKGROUND:

The accurate detection of multidrug-resistant tuberculosis (MDR-TB) is critical for the application of appropriate patient treatment and prevention of transmission of drug-resistant Mycobacterium tuberculosis isolates. The goal of this study was to evaluate the correlation between phenotypic and molecular techniques for drug-resistant tuberculosis diagnostics. Molecular techniques used were the line probe assay genotype MTBDRplus and the recently described tuberculosis-spoligo-rifampin-isoniazid typing (TB-SPRINT) bead-based assay. Conventional drug susceptibility testing (DST) was done on a BACTECTM MGIT 960 TB.

METHOD:

We studied 80 M. tuberculosis complex (MTC) clinical isolates from Minas Gerais state, of which conventional DST had classified 60 isolates as MDR and 20 as drug susceptible.

FINDINGS:

Among the 60 MDR-TB isolates with MGIT as a reference, sensitivity, specificity, accuracy, and kappa for rifampicin (RIF) resistance using TB-SPRINT and MTBDRplus, were 96.7% versus 93.3%, 100.0% versus 100.0%, 97.5% versus 95.0% and 0.94 versus 0.88, respectively. Similarly, the sensitivity, specificity, accuracy, and kappa for isoniazid (INH) resistance were 85.0% and 83.3%, 100.0% and 100.0%, 88.8% and 87.5% and 0.74 and 0.71 for both tests, respectively. Finally, the sensitivity, specificity, accuracy, and kappa for MDR-TB were 85.0% and 83.3%, 100.0% and 100.0%, 88.8% and 87.5% and 0.74 and 0.71 for both tests, respectively. MAIN

CONCLUSIONS:

Both methods exhibited a good correlation with the conventional DST. We suggest estimating the cost-effectiveness of MTBDRplus and TB-SPRINT in Brazil.
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Texto completo: Disponible Colección: Bases de datos internacionales Contexto: OBJETIVO DE DESARROLLO SOSTENIBLE 3 - ODS3 / Enfermedades desatendidas Tema en salud: Meta 3.3: Poner fin a las enfermedades desatendidas y detener enfermedades transmisibles / Tuberculosis Base de datos: MEDLINE Asunto principal: Técnicas Bacteriológicas / Tuberculosis Resistente a Múltiples Medicamentos / Mycobacterium tuberculosis Límite: Humanos País/Región como asunto: America del Sur / Brasil Idioma: Inglés Revista: Mem Inst Oswaldo Cruz Año: 2017 Tipo del documento: Artículo País de afiliación: Brasil