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Determination and optimization of a strong promoter element from Bacillus amyloliquefaciens by using a promoter probe vector.
Liao, Yuling; Wang, Bin; Ye, Yanrui; Pan, Li.
Afiliación
  • Liao Y; School of Bioscience and Bioengineering, South China University of Technology, Guangzhou, 510006, Guangdong, People's Republic of China.
  • Wang B; Star Lake Bioscience Co., Inc, Zhaoqing, 526060, Guangdong, People's Republic of China.
  • Ye Y; School of Bioscience and Bioengineering, South China University of Technology, Guangzhou, 510006, Guangdong, People's Republic of China.
  • Pan L; School of Bioscience and Bioengineering, South China University of Technology, Guangzhou, 510006, Guangdong, People's Republic of China. esyanruiye@scut.edu.cn.
Biotechnol Lett ; 40(1): 119-126, 2018 Jan.
Article en En | MEDLINE | ID: mdl-29101598
ABSTRACT

OBJECTIVE:

To construct a promoter probe vector, pBE-bgaB, to screen strong promoters from Bacillus amyloliquefaciens.

RESULTS:

266 colonies containing active promoter elements from the genomic DNA of B. amyloliquefaciens were identified. Among these, promoter P41 exhibited the strongest ß-Gal activity in Escherichia coli and B. amyloliquefaciens. Sequence analysis showed that promoter P41 contained P ykuN , a ykuN gene encoding flavodoxin. Optimization of the ribosome-binding site from P41 to P382 improved ß-Gal activity by ~ 200%.

CONCLUSION:

A new strong promoter for protein expression and genetic engineering of Bacillus species.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Pruebas Genéticas / Regiones Promotoras Genéticas / Beta-Galactosidasa / Bacillus amyloliquefaciens Idioma: En Revista: Biotechnol Lett Año: 2018 Tipo del documento: Article
Texto completo
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XML
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Pruebas Genéticas / Regiones Promotoras Genéticas / Beta-Galactosidasa / Bacillus amyloliquefaciens Idioma: En Revista: Biotechnol Lett Año: 2018 Tipo del documento: Article