Changes in the expression and localization of signaling molecules in mouse facial motor neurons during regeneration of facial nerves.
J Chem Neuroanat
; 88: 13-21, 2018 03.
Article
en En
| MEDLINE
| ID: mdl-29113945
ABSTRACT
After injury, peripheral axons usually re-extend toward their target, and neuronal functions recover. Previous studies have reported that expression of various molecules are transiently altered in motor neurons after nerve injury, but the time course of these changes and their relationship with functional recovery have not been clearly demonstrated. We used the mouse facial nerve transection and suturing model, and examined the changes in expression of five molecules, choline acetyl transferase (ChAT), galanin, calcitonin gene-related protein (CGRP), gephyrin, and potassium chloride co-transporter 2 (KCC2) in the facial motor neurons after surgery until recovery. Number of ChAT-positive neurons was markedly decreased at days 3 and 7, and recovered to the normal level by day 60, when facial motor functions recovered. Localization of two neuropeptides, CGRP and galanin, was increased in the perikarya and axons during regeneration, and returned to the normal levels by days 60 and 28, respectively. Expression of two postsynaptic elements of γ-amino butyric acid synapses, gephyrin and KCC2, was decreased at days 3 and 7, and recovered by day 60. These results suggest that ChAT, CGRP, and KCC2 may be objective indicators of regeneration, and altering their expression may be related to the functional recovery and axonal re-extension.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Péptido Relacionado con Gen de Calcitonina
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Colina O-Acetiltransferasa
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Simportadores
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Nervio Facial
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Neuronas Motoras
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Regeneración Nerviosa
Límite:
Animals
Idioma:
En
Revista:
J Chem Neuroanat
Asunto de la revista:
ANATOMIA
/
NEUROLOGIA
/
QUIMICA
Año:
2018
Tipo del documento:
Article
País de afiliación:
Corea del Sur