Glutamine protection in an experimental model of acetaminophen nephrotoxicity.
Can J Physiol Pharmacol
; 96(4): 366-371, 2018 Apr.
Article
en En
| MEDLINE
| ID: mdl-29120676
ABSTRACT
Acetaminophen (APAP) is a widely prescribed analgesic and antipyretic drug. In the present work, we studied the effects of glutamine (Gln) in an in vivo model of APAP-induced nephrotoxicity in male Wistar rats. Renal function, histological characteristics, and Na+,K+-ATPase cortical abundance and distribution were analyzed. The appearance of HSP70 and actin in urine was also evaluated. Myeloperoxidase (MPO) activity in cortical tissue was measured as an index of the inflammatory response. Gln administration 30 min before APAP protected from the renal functional and histological damage promoted by APAP. Rats that received the dual treatment Gln and APAP (Gln/APAP) showed the same level of Na+,K+-ATPase cortical induction as APAP-treated animals, but the enzyme maintained its normal basolateral localization. HSP70 abundance was increased up to the same level in the Gln, APAP, and Gln/APAP groups. Urinary HSP70 and actin were detected only in the APAP-treated animals, reinforcing the protection of renal tubular integrity afforded by the Gln pretreatment. Gln pretreatment also protected from the increment in MPO activity promoted by APAP. Our results support the idea that Gln pretreatment could be a therapeutic option to prevent APAP-induced renal injury.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Sustancias Protectoras
/
Glutamina
/
Enfermedades Renales
/
Acetaminofén
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
En
Revista:
Can J Physiol Pharmacol
Año:
2018
Tipo del documento:
Article
País de afiliación:
Argentina