Analysis of XFEL serial diffraction data from individual crystalline fibrils.
IUCrJ
; 4(Pt 6): 795-811, 2017 Nov 01.
Article
en En
| MEDLINE
| ID: mdl-29123682
ABSTRACT
Serial diffraction data collected at the Linac Coherent Light Source from crystalline amyloid fibrils delivered in a liquid jet show that the fibrils are well oriented in the jet. At low fibril concentrations, diffraction patterns are recorded from single fibrils; these patterns are weak and contain only a few reflections. Methods are developed for determining the orientation of patterns in reciprocal space and merging them in three dimensions. This allows the individual structure amplitudes to be calculated, thus overcoming the limitations of orientation and cylindrical averaging in conventional fibre diffraction analysis. The advantages of this technique should allow structural studies of fibrous systems in biology that are inaccessible using existing techniques.
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1
Colección:
01-internacional
Base de datos:
MEDLINE
Idioma:
En
Revista:
IUCrJ
Año:
2017
Tipo del documento:
Article
País de afiliación:
Nueva Zelanda