Protective effects of fucoidan against hyperoxic lung injury via the ERK signaling pathway.
Mol Med Rep
; 17(1): 1813-1818, 2018 Jan.
Article
en En
| MEDLINE
| ID: mdl-29138816
ABSTRACT
High oxygen mechanical ventilation is widely used to treat various lung diseases; however, it may result in hyperoxia, which induces inflammation and lung injury. Fucoidan is an extract of the seaweed Fucus vesiculosus, which has previously been reported to exert effects against diabetic nephropathy. The present study is the first, to the best of our knowledge, to investigate the protective effects of fucoidan against hyperoxic lung injury. Balb/c mice were ventilated with 100% oxygen, with or without the atomization inhalation of fucoidan, for 36 h. Hyperoxia reduced the body weight and increased the relative lung weight of the mice. In addition, cell quantity and differentiation were determined using a hemocytometer, hyperoxia increased the total number of cells, and the number of macrophages, neutrophils and lymphocytes in the bronchoalveolar lavage fluid. Reverse transcriptionquantitative polymerase chain reaction (RTqPCR) demonstrated that hyperoxia also increased the mRNA expression levels of cluster of differentiation (CD)68, F4/80, CD64 and CD19 in lung tissue, and induced lung morphological alterations. Furthermore, western blotting assay demonstrated that hyperoxia increased the expression levels of interleukin (IL)1, IL6 and tumor necrosis factor (TNF)α, and the phosphorylation of extracellular signalregulated kinase (ERK)1/2. Conversely, hyperoxiainduced inflammation and morphological alterations were significantly attenuated in the mice treated with fucoidan. Atomization inhalation of fucoidan also reduced the hyperoxiainduced expression of IL1, IL6 and TNFα, and the phosphorylation of ERK1/2. These findings suggested that fucoidan may attenuate hyperoxic lung injury via the ERK1/2 signaling pathway.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Polisacáridos
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Hiperoxia
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Sistema de Señalización de MAP Quinasas
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Lesión Pulmonar Inducida por Ventilación Mecánica
Límite:
Animals
Idioma:
En
Revista:
Mol Med Rep
Año:
2018
Tipo del documento:
Article
Pais de publicación:
GR
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GRECIA
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GREECE
/
GRÉCIA