Vasohibins encode tubulin detyrosinating activity.
Science
; 358(6369): 1453-1456, 2017 12 15.
Article
en En
| MEDLINE
| ID: mdl-29146869
ABSTRACT
Tubulin is subjected to a number of posttranslational modifications to generate heterogeneous microtubules. The modifications include removal and ligation of the C-terminal tyrosine of âº-tubulin. The enzymes responsible for detyrosination, an activity first observed 40 years ago, have remained elusive. We applied a genetic screen in haploid human cells to find regulators of tubulin detyrosination. We identified SVBP, a peptide that regulates the abundance of vasohibins (VASH1 and VASH2). Vasohibins, but not SVBP alone, increased detyrosination of âº-tubulin, and purified vasohibins removed the C-terminal tyrosine of âº-tubulin. We found that vasohibins play a cell type-dependent role in detyrosination, although cells also contain an additional detyrosinating activity. Thus, vasohibins, hitherto studied as secreted angiogenesis regulators, constitute a long-sought missing link in the tubulin tyrosination cycle.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Tirosina
/
Tubulina (Proteína)
/
Carboxipeptidasas
/
Proteínas Portadoras
/
Proteínas de Ciclo Celular
/
Proteínas Angiogénicas
Límite:
Humans
Idioma:
En
Revista:
Science
Año:
2017
Tipo del documento:
Article
País de afiliación:
Países Bajos
Pais de publicación:
EEUU
/
ESTADOS UNIDOS
/
ESTADOS UNIDOS DA AMERICA
/
EUA
/
UNITED STATES
/
UNITED STATES OF AMERICA
/
US
/
USA