RNA Interference-Mediated Gene Silencing by Branched Tripodal RNAs Does Not Require Dicer Processing.
Nucleic Acid Ther
; 28(1): 44-49, 2018 02.
Article
en En
| MEDLINE
| ID: mdl-29195056
ABSTRACT
Specific gene silencing through RNA interference (RNAi) holds great promise as the next-generation therapeutic development platform. Previously, we have shown that branched, tripodal interfering RNA (tiRNA) structures could simultaneously trigger RNAi-mediated gene silencing of three target genes with 38 nt-long guide strands associated with Argonaute 2. Herein, we show that the branched RNA structure can trigger effective gene silencing in Dicer knockout cell line, demonstrating that the Dicer-mediated processing is not required for tiRNA activity. The finding of this study confirms the flexibility of the structure of RNAi triggers as well as the length of the guide strand in RNAi-mediated gene silencing.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
ARN Guía de Kinetoplastida
/
ARN Interferente Pequeño
/
Interferencia de ARN
/
Ribonucleasa III
/
ARN Helicasas DEAD-box
/
Proteínas Argonautas
Límite:
Humans
Idioma:
En
Revista:
Nucleic Acid Ther
Año:
2018
Tipo del documento:
Article