The precise subcellular localization of Dlg in the Drosophila larva body wall using improved pre-embedding immuno-EM.
J Neurosci Res
; 96(3): 467-480, 2018 03.
Article
en En
| MEDLINE
| ID: mdl-29231975
ABSTRACT
Discs-large (Dlg) plays important roles in nerve tissue and epithelial tissue in Drosophila. However, the precise positioning of Dlg in the neuromuscular junction remains to be confirmed using an optimized labeling method. In this study, we improved the method of pre-embedding immunogold electron microscopy without the osmic tetroxide procedure, and we found that Lowicryl K4 M resin and low temperature helped to preserve the authenticity of the labeling signal with relatively good contrast. Dlg was strongly expressed in the entire subsynaptic reticulum (SSR) membrane of type Ib boutons, expressed in parts of the SSR membrane of type Is boutons, weakly expressed in axon terminals and axons, and not expressed in pre- or postsynaptic membranes of type Is boutons. In muscle cells and stratum corneum cells, Dlg was expressed both in the cytoplasm and in organelles with biomembranes. The precise location of Dlg in SSR membranes, rather than in postsynaptic membranes, shows that Dlg, with its multiple domains, acts as a remote or indirect regulator in postsynaptic signal transduction.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Inmunohistoquímica
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Microscopía Inmunoelectrónica
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Proteínas de Drosophila
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Proteínas Supresoras de Tumor
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Drosophila
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Larva
Límite:
Animals
Idioma:
En
Revista:
J Neurosci Res
Año:
2018
Tipo del documento:
Article
País de afiliación:
China