A far-red fluorescent probe based on a phospha-fluorescein scaffold for cytosolic calcium imaging.
Chem Commun (Camb)
; 54(3): 299-302, 2018 Jan 02.
Article
en En
| MEDLINE
| ID: mdl-29239411
ABSTRACT
The far-red emissive fluorescent probe CaPF-1 based on a phospha-fluorescein scaffold enables the detection of cytosolic calcium ions in living cells. The probe can be excited in the red region (λabs = 636 nm) and exhibits a sufficiently high fluorescence turn-on response in the far-red region (λem = 663 nm) upon complexation with calcium ions. The hydrophilic and anionic characteristics of this phospha-fluorescein fluorophore allowed the cytosolic localization of CaPF-1. Moreover, it was possible to visualize histamine-induced calcium oscillation in HeLa cells using CaPF-1.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Calcio
/
Óxidos P-Cíclicos
/
Fluoresceínas
/
Colorantes Fluorescentes
Límite:
Humans
Idioma:
En
Revista:
Chem Commun (Camb)
Asunto de la revista:
QUIMICA
Año:
2018
Tipo del documento:
Article
País de afiliación:
Japón