Improved detectability of sex steroids from frozen sections of breast cancer tissue using GC-triple quadrupole-MS.
J Steroid Biochem Mol Biol
; 178: 185-192, 2018 04.
Article
en En
| MEDLINE
| ID: mdl-29269263
ABSTRACT
Sex steroids in clinical endocrinology have been mainly investigated with peripheral blood and urine samples, while there is limited information regarding the local levels within tissues. To improve analytical properties of sex steroids from trace amounts of tissue samples, two-phase extractive ethoxycarbonlyation and subsequent pentafluoropropionyl derivatization coupled to gas chromatography-tandem mass spectrometry (GC-MS/MS) was developed. The optimized analytical conditions led to excellent chromatographic separation of 15 estrogens, 6 androgens, and 2 progestins. The quantitative results were calculated based on in-house control samples as the steroid-free tissues, and the precision and accuracy were 4.2%-26.8% and 90.8%-116.4%, respectively. The on-column limit of quantification was from 180â¯fg to 0.5â¯pg for androgens and estrogens, and 1.25â¯pg for progestins, which were found to be linear (r2â¯>â¯0.990). The validated method was then applied to quantify 7 sex steroids from three 100-µm-thick frozen breast tissue slices from postmenopausal patients with breast cancer. This is the first report on the improved GC-MS/MS method for the detection of androgens and pregnenolone from breast cancer tissues, and it can be a useful technique to measure the local levels of sex steroids, thus, enhancing our understanding of the pathophysiological significances of steroidogenesis.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Progestinas
/
Mama
/
Neoplasias de la Mama
/
Estrógenos
/
Espectrometría de Masas en Tándem
/
Andrógenos
/
Cromatografía de Gases y Espectrometría de Masas
Tipo de estudio:
Observational_studies
Límite:
Female
/
Humans
Idioma:
En
Revista:
J Steroid Biochem Mol Biol
Asunto de la revista:
BIOLOGIA MOLECULAR
/
BIOQUIMICA
Año:
2018
Tipo del documento:
Article