Identifying Novel Enhancer Elements with CRISPR-Based Screens.
ACS Chem Biol
; 13(2): 326-332, 2018 02 16.
Article
en En
| MEDLINE
| ID: mdl-29300083
ABSTRACT
Enhancers control the spatiotemporal expression of genes and are essential for encoding differentiation and development. Since their discovery more than three decades ago, researchers have largely studied enhancers removed from their genomic context. The recent adaptation of CRISPR/Cas9 to genome editing in higher organisms now allows researchers to perturb and test these elements in their genomic context, through both mutation and epigenetic modulation. In this Perspective, we discuss recent advances in scanning noncoding regions of the genome for enhancer activity using CRISPR-based tools.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Elementos de Facilitación Genéticos
/
Sistemas CRISPR-Cas
/
Edición Génica
Límite:
Humans
Idioma:
En
Revista:
ACS Chem Biol
Año:
2018
Tipo del documento:
Article
País de afiliación:
Estados Unidos