TPXL-1 activates Aurora A to clear contractile ring components from the polar cortex during cytokinesis.
J Cell Biol
; 217(3): 837-848, 2018 03 05.
Article
en En
| MEDLINE
| ID: mdl-29311228
ABSTRACT
During cytokinesis, a signal from the central spindle that forms between the separating anaphase chromosomes promotes the accumulation of contractile ring components at the cell equator, while a signal from the centrosomal microtubule asters inhibits accumulation of contractile ring components at the cell poles. However, the molecular identity of the inhibitory signal has remained unknown. To identify molecular components of the aster-based inhibitory signal, we developed a means to monitor the removal of contractile ring proteins from the polar cortex after anaphase onset. Using this assay, we show that polar clearing is an active process that requires activation of Aurora A kinase by TPXL-1. TPXL-1 concentrates on astral microtubules coincident with polar clearing in anaphase, and its ability to recruit Aurora A and activate its kinase activity are essential for clearing. In summary, our data identify Aurora A kinase as an aster-based inhibitory signal that restricts contractile ring components to the cell equator during cytokinesis.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Transducción de Señal
/
Proteínas Portadoras
/
Caenorhabditis elegans
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Proteínas de Caenorhabditis elegans
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Citocinesis
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Aurora Quinasa A
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Anafase
Límite:
Animals
Idioma:
En
Revista:
J Cell Biol
Año:
2018
Tipo del documento:
Article
País de afiliación:
Alemania