Evaluation of the Efficiency of Lytic Mycobacteriophage D29 on the Model of M. tuberculosis-Infected Macrophage RAW 264 Cell Line.
Bull Exp Biol Med
; 164(3): 344-346, 2018 Jan.
Article
en En
| MEDLINE
| ID: mdl-29313233
ABSTRACT
Culture of mouse macrophages (RAW 264.7 ATCC strain) in wells of a 6-well plate was infected with M. tuberculosis in proportion of 15 mycobacteria per one macrophage and then treated with a lytic strain of mycobacteriophage D29. Antibacterial efficacy of mycobacteriophages was studied using D29 phage (activity 108 plaque-forming units/ml) previously purified by ion exchange chromatography. After single and double 24-h treatment, the lysed cultures of macrophages were inoculated onto Middlebrook 7H10 agar medium. The number of mycobacterial colonies in control and test wells (at least 3 wells in each group) was 300.178±12.500 and 36.0±5.4, respectively (p<0.01).
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Lisogenia
/
Micobacteriófagos
/
Mycobacterium tuberculosis
Límite:
Animals
Idioma:
En
Revista:
Bull Exp Biol Med
Año:
2018
Tipo del documento:
Article
País de afiliación:
Rusia