Carbamoyl-phosphate synthetases from Neurospora crassa. Immunological relatedness of the enzymes from Neurospora, bacteria, yeast, and mammals.
J Biol Chem
; 260(26): 14355-62, 1985 Nov 15.
Article
en En
| MEDLINE
| ID: mdl-2932445
ABSTRACT
Neurospora crassa contains two carbamoyl-phosphate synthetases a mitochondrial enzyme (CPS-A) which supplies carbamoyl phosphate for arginine biosynthesis, and a nuclear enzyme whose product is used for the synthesis of pyrimidines. We have prepared antiserum against a highly purified preparation of the large subunit of CPS-A and have used the antiserum to demonstrate that the large subunit is, like most mitochondrially localized proteins, initially synthesized as a higher molecular weight precursor. The CPS-A antiserum cross-reacts with the nuclear enzyme, allowing us to identify the product of the complex N. crassa pyr-3 genetic locus as a protein with a subunit molecular weight of 180,000. Finally, we have found that the CPS-A antiserum also cross-reacts with carbamoyl-phosphate synthetases from bacteria, yeast, and mammals. The immunological relatedness of carbamoyl-phosphate synthetases from such diverse species suggests that the protein sequences required for carbamoyl phosphate production have been highly conserved during the course of evolution.
Buscar en Google
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Carbamoil-Fosfato Sintasa (Amoniaco)
/
Ligasas
/
Mitocondrias
/
Neurospora
/
Neurospora crassa
Límite:
Animals
Idioma:
En
Revista:
J Biol Chem
Año:
1985
Tipo del documento:
Article