An orientation-independent DIC microscope allows high resolution imaging of epithelial cell migration and wound healing in a cnidarian model.
J Microsc
; 270(3): 290-301, 2018 06.
Article
en En
| MEDLINE
| ID: mdl-29345317
ABSTRACT
Epithelial cell dynamics can be difficult to study in intact animals or tissues. Here we use the medusa form of the hydrozoan Clytia hemisphaerica, which is covered with a monolayer of epithelial cells, to test the efficacy of an orientation-independent differential interference contrast microscope for in vivo imaging of wound healing. Orientation-independent differential interference contrast provides an unprecedented resolution phase image of epithelial cells closing a wound in a live, nontransgenic animal model. In particular, the orientation-independent differential interference contrast microscope equipped with a 40x/0.75NA objective lens and using the illumination light with wavelength 546 nm demonstrated a resolution of 460 nm. The repair of individual cells, the adhesion of cells to close a gap, and the concomitant contraction of these cells during closure is clearly visualized.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Cicatrización de Heridas
/
Movimiento Celular
/
Hidrozoos
/
Células Epiteliales
/
Microscopía Intravital
/
Microscopía de Interferencia
Límite:
Animals
Idioma:
En
Revista:
J Microsc
Año:
2018
Tipo del documento:
Article
País de afiliación:
Estados Unidos