[Activity of membrane-bound enzymes, indices of metabolism in the cytoplasm and various physico-chemical properties of erythrocytes with increased cholesterol level]. / Aktivnost' membranno-sviazannykh fermentov, pokazateli metabolizma v tsitoplazme i nekotorye fiziko-khimicheskie svoistva éritrotsitov s povyshennym soderzhaniem kholesterina.

ABSTRACT

Activity of membrane-bound enzymes, passive penetration of ions in dose-dependent loading with cholesterol, effect of cholesterol high concentrations on the metabolic patterns in cytosol, viscosity of cell suspension were studied in erythrocytes. Passive cotransport of H+ and Cl- ions via erythrocyte membrane was increased with augmentation in viscosity of the cell suspension. After loading with cholesterol activity of acetylcholinesterase was increased while ATPase and glyceraldehyde-3-phosphate dehydrogenase activities were decreased. The alteration in the enzymatic activity occurred on those sides of the membranes, where these enzymes were localized. Activity of lactate dehydrogenase was decreased in cytoplasm of erythrocytes. The alterations detected may be important in development of ischemic syndrome in hyperlipoproteinemia.