Pectin characterisation using size exclusion chromatography: A comparison of ELS and RI detection.
Food Chem
; 252: 271-276, 2018 Jun 30.
Article
en En
| MEDLINE
| ID: mdl-29478541
ABSTRACT
A high-performance size-exclusion chromatography (HPSEC) method coupled to Evaporative Light Scattering (ELS) and Refractive Index (RI) detectors were evaluated and compared for the molecular mass (Mw) estimation of pectin in a wide range (0.342-805â¯kDa). Instrumental parameters of the ELSD were optimised by Response Surface Methodology (RSM) being 73⯰C the evaporator temperature and 0.9â¯mL/min the air flow rate. The linear range for the ELSD concentration response was wider (10-2250â¯mg/L) and better (R2â¯=â¯0.985) than RID (10-1500â¯mg/L; R2â¯=â¯0.875). The limits of detection (LOD) and quantitation (LOQ) for all pullulans hardly changed in ELSD (LOD 1.22-1.99â¯mg/L; LOQ 4.07-6.63â¯mg/L); however, RID showed huge variations (LOD 0.49-10.41â¯mg/L; LOQ 1.64-34.70â¯mg/L), which increased with the Mw. In general, responses of both detectors were similar for the Mw estimation, although pectin characterisation with HPSEC-ELSD exhibited better results in the lowest Mw compounds.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Extractos Vegetales
/
Cromatografía en Gel
/
Pectinas
Tipo de estudio:
Diagnostic_studies
Idioma:
En
Revista:
Food Chem
Año:
2018
Tipo del documento:
Article
País de afiliación:
España
Pais de publicación:
ENGLAND
/
ESCOCIA
/
GB
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GREAT BRITAIN
/
INGLATERRA
/
REINO UNIDO
/
SCOTLAND
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UK
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UNITED KINGDOM