Integrase-Mediated Recombination of the bel-1 Gene Cassette Encoding the Extended-Spectrum ß-Lactamase BEL-1.
Antimicrob Agents Chemother
; 62(5)2018 05.
Article
en En
| MEDLINE
| ID: mdl-29483118
ABSTRACT
Integrons are genetic elements that can acquire and rearrange gene cassettes. The blaBEL-1 gene encodes an extended-spectrum ß-lactamase, BEL-1, that is present at the second position of the variable region of class 1 integrons identified in Pseudomonas aeruginosa The mobility of the bel-1 gene cassette was analyzed under physiological conditions and with the integrase gene being overexpressed. Cassette mobility in Escherichia coli was detected by excision/integration into the recipient integron In3 on the conjugative plasmid R388 with the overproduced integrase. Despite several antibiotic pressures, the bel-1 cassette remained at the second position in the integron, highlighting its stability in P. aeruginosa Overexpression of the integrase gene in E. coli induced bel-1 cassette recombination. However, cassettes containing two genes (blaBEL-1 and smr2 or blaBEL-1 and aacA4) were excised, suggesting that the bel-1 cassette attC site was defective. We show that bel-1 is a stable gene cassette under physiological growth conditions, irrespective of the selective antibiotic pressure, that may be mobilized upon overexpression of the integrase gene.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Pseudomonas aeruginosa
/
Beta-Lactamasas
/
Integrasas
Tipo de estudio:
Prognostic_studies
Idioma:
En
Revista:
Antimicrob Agents Chemother
Año:
2018
Tipo del documento:
Article
País de afiliación:
Francia