Molecular Requirements for Self-Interaction of the Respiratory Syncytial Virus Matrix Protein in Living Mammalian Cells.
Viruses
; 10(3)2018 03 03.
Article
en En
| MEDLINE
| ID: mdl-29510513
ABSTRACT
Respiratory syncytial virus (RSV) is an important human pathogen, which infects respiratory tract epithelial cells causing bronchiolitis and pneumonia in children and the elderly. Recent studies have linked RSV matrix (M) ability to self-interaction and viral budding. However, RSV M has been crystalized both as a monomer and a dimer, and no formal proof exists to date that it forms dimers in cells. Here, by using a combination of confocal laser scanning microscopy and bioluminescent resonant energy transfer applied to differently tagged deletion mutants of RSV M, we show that the protein can self-interact in living mammalian cells and that both the N and C-terminus of the protein are strictly required for the process, consistent with the reported dimeric crystal structure.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Virus Sincitiales Respiratorios
/
Proteínas de la Matriz Viral
/
Infecciones por Virus Sincitial Respiratorio
/
Multimerización de Proteína
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Viruses
Año:
2018
Tipo del documento:
Article
País de afiliación:
Italia