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Retrograde Neuroanatomical Tracing of Phrenic Motor Neurons in Mice.
Vandeweerd, Jean-Michel; Hontoir, Fanny; De Knoop, Alexis; De Swert, Kathleen; Nicaise, Charles.
Afiliación
  • Vandeweerd JM; URVI, NARILIS, Université de Namur.
  • Hontoir F; URVI, NARILIS, Université de Namur.
  • De Knoop A; URVI, NARILIS, Université de Namur.
  • De Swert K; URPhyM, NARILIS, Université de Namur.
  • Nicaise C; URPhyM, NARILIS, Université de Namur; charles.nicaise@unamur.be.
J Vis Exp ; (132)2018 02 22.
Article en En | MEDLINE | ID: mdl-29553523
Phrenic motor neurons are cervical motor neurons originating from C3 to C6 levels in most mammalian species. Axonal projections converge into phrenic nerves innervating the respiratory diaphragm. In spinal cord slices, phrenic motor neurons cannot be identified from other motor neurons on morphological or biochemical criteria. We provide the description of procedures for visualizing phrenic motor neuron cell bodies in mice, following intrapleural injections of cholera toxin subunit beta (CTB) conjugated to a fluorophore. This fluorescent neuroanatomical tracer has the ability to be caught up at the diaphragm neuromuscular junction, be carried retrogradely along the phrenic axons and reach the phrenic cell bodies. Two methodological approaches of intrapleural CTB delivery are compared: transdiaphragmatic versus transthoracic injections. Both approaches are successful and result in similar number of CTB-labeled phrenic motor neurons. In conclusion, these techniques can be applied to visualize or quantify the phrenic motor neurons in various experimental studies such as those focused on the diaphragm-phrenic circuitry.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Nervio Frénico / Neuronas Motoras Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: J Vis Exp Año: 2018 Tipo del documento: Article Pais de publicación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Nervio Frénico / Neuronas Motoras Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: J Vis Exp Año: 2018 Tipo del documento: Article Pais de publicación: Estados Unidos