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Genomic characterization of malonate positive Cronobacter sakazakii serotype O:2, sequence type 64 strains, isolated from clinical, food, and environment samples.
Gopinath, Gopal R; Chase, Hannah R; Gangiredla, Jayanthi; Eshwar, Athmanya; Jang, Hyein; Patel, Isha; Negrete, Flavia; Finkelstein, Samantha; Park, Eunbi; Chung, TaeJung; Yoo, YeonJoo; Woo, JungHa; Lee, YouYoung; Park, Jihyeon; Choi, Hyerim; Jeong, Seungeun; Jun, Soyoung; Kim, Mijeong; Lee, Chaeyoon; Jeong, HyeJin; Fanning, Séamus; Stephan, Roger; Iversen, Carol; Reich, Felix; Klein, Günter; Lehner, Angelika; Tall, Ben D.
Afiliación
  • Gopinath GR; 1Center of Food Safety and Applied Nutrition, U. S. Food and Drug Administration, Laurel, MD 20708 USA.
  • Chase HR; 1Center of Food Safety and Applied Nutrition, U. S. Food and Drug Administration, Laurel, MD 20708 USA.
  • Gangiredla J; 1Center of Food Safety and Applied Nutrition, U. S. Food and Drug Administration, Laurel, MD 20708 USA.
  • Eshwar A; 2Institute for Food Safety and Hygiene, University of Zurich, Zurich, Switzerland.
  • Jang H; 1Center of Food Safety and Applied Nutrition, U. S. Food and Drug Administration, Laurel, MD 20708 USA.
  • Patel I; 1Center of Food Safety and Applied Nutrition, U. S. Food and Drug Administration, Laurel, MD 20708 USA.
  • Negrete F; 1Center of Food Safety and Applied Nutrition, U. S. Food and Drug Administration, Laurel, MD 20708 USA.
  • Finkelstein S; 1Center of Food Safety and Applied Nutrition, U. S. Food and Drug Administration, Laurel, MD 20708 USA.
  • Park E; 1Center of Food Safety and Applied Nutrition, U. S. Food and Drug Administration, Laurel, MD 20708 USA.
  • Chung T; 1Center of Food Safety and Applied Nutrition, U. S. Food and Drug Administration, Laurel, MD 20708 USA.
  • Yoo Y; 1Center of Food Safety and Applied Nutrition, U. S. Food and Drug Administration, Laurel, MD 20708 USA.
  • Woo J; 1Center of Food Safety and Applied Nutrition, U. S. Food and Drug Administration, Laurel, MD 20708 USA.
  • Lee Y; 1Center of Food Safety and Applied Nutrition, U. S. Food and Drug Administration, Laurel, MD 20708 USA.
  • Park J; 1Center of Food Safety and Applied Nutrition, U. S. Food and Drug Administration, Laurel, MD 20708 USA.
  • Choi H; 1Center of Food Safety and Applied Nutrition, U. S. Food and Drug Administration, Laurel, MD 20708 USA.
  • Jeong S; 1Center of Food Safety and Applied Nutrition, U. S. Food and Drug Administration, Laurel, MD 20708 USA.
  • Jun S; 1Center of Food Safety and Applied Nutrition, U. S. Food and Drug Administration, Laurel, MD 20708 USA.
  • Kim M; 1Center of Food Safety and Applied Nutrition, U. S. Food and Drug Administration, Laurel, MD 20708 USA.
  • Lee C; 1Center of Food Safety and Applied Nutrition, U. S. Food and Drug Administration, Laurel, MD 20708 USA.
  • Jeong H; 1Center of Food Safety and Applied Nutrition, U. S. Food and Drug Administration, Laurel, MD 20708 USA.
  • Fanning S; 3UCD Centre for Food Safety, School of Public Health, Physiotherapy & Population Science, University College, Dublin & WHO Collaborating Centre for Cronobacter, Belfield, Dublin 4, Ireland.
  • Stephan R; 2Institute for Food Safety and Hygiene, University of Zurich, Zurich, Switzerland.
  • Iversen C; 2Institute for Food Safety and Hygiene, University of Zurich, Zurich, Switzerland.
  • Reich F; 3UCD Centre for Food Safety, School of Public Health, Physiotherapy & Population Science, University College, Dublin & WHO Collaborating Centre for Cronobacter, Belfield, Dublin 4, Ireland.
  • Klein G; 4Institute for Food Quality and Safety, University of Veterinary Medicine Hannover, Bischofsholer Damm 15, 30173 Hannover, Germany.
  • Lehner A; 4Institute for Food Quality and Safety, University of Veterinary Medicine Hannover, Bischofsholer Damm 15, 30173 Hannover, Germany.
  • Tall BD; 2Institute for Food Safety and Hygiene, University of Zurich, Zurich, Switzerland.
Gut Pathog ; 10: 11, 2018.
Article en En | MEDLINE | ID: mdl-29556252
ABSTRACT

BACKGROUND:

Malonate utilization, an important differential trait, well recognized as being possessed by six of the seven Cronobacter species is thought to be largely absent in Cronobacter sakazakii (Csak). The current study provides experimental evidence that confirms the presence of a malonate utilization operon in 24 strains of sequence type (ST) 64, obtained from Europe, Middle East, China, and USA; it offers explanations regarding the genomic diversity and phylogenetic relatedness among these strains, and that of other C. sakazakii strains.

RESULTS:

In this study, the presence of a malonate utilization operon in these strains was initially identified by DNA microarray analysis (MA) out of a pool of 347 strains obtained from various surveillance studies involving clinical, spices, milk powder sources and powdered infant formula production facilities in Ireland and Germany, and dried dairy powder manufacturing facilities in the USA. All ST64 C. sakazakii strains tested could utilize malonate. Zebrafish embryo infection studies showed that C. sakazakii ST64 strains are as virulent as other Cronobacter species. Parallel whole genome sequencing (WGS) and MA showed that the strains phylogenetically grouped as a separate clade among the Csak species cluster. Additionally, these strains possessed the Csak O2 serotype. The nine-gene, ~ 7.7 kbp malonate utilization operon was located in these strains between two conserved flanking genes, gyrB and katG. Plasmidotyping results showed that these strains possessed the virulence plasmid pESA3, but in contrast to the USA ST64 Csak strains, ST64 Csak strains isolated from sources in Europe and the Middle East, did not possess the type six secretion system effector vgrG gene.

CONCLUSIONS:

Until this investigation, the presence of malonate-positive Csak strains, which are associated with foods and clinical cases, was under appreciated. If this trait was used solely to identify Cronobacter strains, many strains would likely be misidentified. Parallel WGS and MA were useful in characterizing the total genome content of these Csak O2, ST64, malonate-positive strains and further provides an understanding of their phylogenetic relatedness among other virulent C. sakazakii strains.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Gut Pathog Año: 2018 Tipo del documento: Article
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Gut Pathog Año: 2018 Tipo del documento: Article