Identification and characterization of cis-regulatory elements 'insulator and repressor' in PPARD gene.
Epigenomics
; 10(5): 613-627, 2018 05.
Article
en En
| MEDLINE
| ID: mdl-29583017
ABSTRACT
AIM:
Identification and functional characterization of cis-regulatory elements in human PPARD gene.METHODS:
We used various bioinformatic tools on the publicly available human genome and Encyclopedia of DNA Elements databases to explore potential cis-regulatory elements in PPARD gene region.RESULTS:
We predicted an insulator and an enhancer element in intron 2 of PPARD gene. Functional characterization using transient transfection, reporter assay and CTCF binding confirmed the insulator status. However, the predicted enhancer element showed repressor/silencer activity. Finally, we observed a potential interaction between these two cis-regulatory elements which is in agreement with 5C-Encyclopedia of DNA Elements data.CONCLUSION:
We report two functionally validated cis-regulatory elements in PPARD gene which will aid in understanding its regulation and role in metabolic functions.Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Genoma Humano
/
Elementos de Facilitación Genéticos
/
Elementos Aisladores
/
PPAR delta
Tipo de estudio:
Diagnostic_studies
Límite:
Humans
Idioma:
En
Revista:
Epigenomics
Año:
2018
Tipo del documento:
Article
País de afiliación:
India