Glucose oxidase assisted visual detection of glucose using oxygen deficient α-MoO3-x nanoflakes.
Mikrochim Acta
; 185(1): 65, 2017 12 18.
Article
en En
| MEDLINE
| ID: mdl-29594588
ABSTRACT
An optical method is described for the quantitation of glucose by using oxygen-deficient α-MoO3-x nanoflakes. It is based on the use of glucose oxidase (GOx) which produces hydrogen peroxide on oxidation of glucose. Hydrogen peroxide then oxidizes the α-MoO3-x nanoflakes, and this results in a visible color change from blue to colorless. The color change can be measured photometrically at 740 nm. The method has a 68 nM detection limit. Graphical Abstract Mechanism of glucose detection using blue colored oxygen deficient 2D α-MoO3-x nanoflakes. Hydrogen peroxide (H2O2) is formed as a by-product in the conversion of glucose to glucono-1,5-lactone by glucose oxidase (GOx). In the presence of H2O2, the oxygen vacancies in α-MoO3-x nanoflakes are filled up, and this leads to the loss of blue color of the nanoflakes because they are converted back to colorless bulk α-MoO3.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Óxidos
/
Oxígeno
/
Técnicas Biosensibles
/
Nanoestructuras
/
Glucosa
/
Glucosa Oxidasa
/
Molibdeno
Tipo de estudio:
Diagnostic_studies
Idioma:
En
Revista:
Mikrochim Acta
Año:
2017
Tipo del documento:
Article
País de afiliación:
Taiwán