Development and validation of a real-time PCR specific for the leukotoxin gene of Bibersteinia trehalosi.
J Vet Diagn Invest
; 30(4): 589-592, 2018 Jul.
Article
en En
| MEDLINE
| ID: mdl-29601780
ABSTRACT
A real-time PCR assay for the leukotoxin gene of Bibersteinia trehalosi was developed and validated to better identify this pathogen, which is a cause of respiratory disease in bighorn sheep. The specificity of the PCR primers was evaluated with DNA from 59 known isolates of the Pasteurellaceae family. For validation, 162 field samples were compared using both the new assay and an indirect method using 2 sets of published protocols. The real-time PCR assay was found to be specific for the leukotoxin gene of B. trehalosi and provides a rapid and direct approach for detecting leukotoxin-producing forms of this organism from samples containing mixed species of leukotoxin-positive Pasteurellaceae.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Gammaproteobacteria
/
Borrego Cimarrón
/
Exotoxinas
/
Reacción en Cadena en Tiempo Real de la Polimerasa
Límite:
Animals
Idioma:
En
Revista:
J Vet Diagn Invest
Asunto de la revista:
MEDICINA VETERINARIA
Año:
2018
Tipo del documento:
Article