Integrating T7 RNA Polymerase and Its Cognate Transcriptional Units for a Host-Independent and Stable Expression System in Single Plasmid.
ACS Synth Biol
; 7(5): 1424-1435, 2018 05 18.
Article
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| MEDLINE
| ID: mdl-29609457
ABSTRACT
Metabolic engineering and synthetic biology usually require universal expression systems for stable and efficient gene expression in various organisms. In this study, a host-independent and stable T7 expression system had been developed by integrating T7 RNA polymerase and its cognate transcriptional units in single plasmid. The expression of T7 RNA polymerase was restricted below its lethal threshold using a T7 RNA polymerase antisense gene cassette, which allowed long periods of cultivation and protein production. In addition, by designing ribosome binding sites, we further tuned the expression capacity of this novel T7 system within a wide range. This host-independent expression system efficiently expressed genes in five different Gram-negative strains and one Gram-positive strain and was also shown to be applicable in a real industrial d- p-hydroxyphenylglycine production system.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Plásmidos
/
Proteínas Virales
/
ARN Polimerasas Dirigidas por ADN
/
Ingeniería Metabólica
/
Glicina
Idioma:
En
Revista:
ACS Synth Biol
Año:
2018
Tipo del documento:
Article
País de afiliación:
China