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Release of Staphylococcus aureus extracellular vesicles and their application as a vaccine platform.
Wang, Xiaogang; Thompson, Christopher D; Weidenmaier, Christopher; Lee, Jean C.
Afiliación
  • Wang X; Division of Infectious Diseases, Department of Medicine, Brigham and Women's Hospital and Harvard Medical School, 181 Longwood Avenue, Boston, MA, 02115, USA.
  • Thompson CD; Division of Infectious Diseases, Department of Medicine, Brigham and Women's Hospital and Harvard Medical School, 181 Longwood Avenue, Boston, MA, 02115, USA.
  • Weidenmaier C; Interfaculty Institute for Microbiology and Infection Medicine, University of Tuebingen, Elfriede Aulhorn Strasse 6, 72076, Tuebingen, Germany.
  • Lee JC; German Center for Infection Research, Partner Site Tuebingen, 72076 Tuebingen, Germany.
Nat Commun ; 9(1): 1379, 2018 04 11.
Article en En | MEDLINE | ID: mdl-29643357
Secretion of extracellular vesicles (EVs), a process common to eukaryotes, archae, and bacteria, represents a secretory pathway that allows cell-free intercellular communication. Microbial EVs package diverse proteins and influence the host-pathogen interaction, but the mechanisms underlying EV production in Gram-positive bacteria are poorly understood. Here we show that EVs purified from community-associated methicillin-resistant Staphylococcus aureus package cytosolic, surface, and secreted proteins, including cytolysins. Staphylococcal alpha-type phenol-soluble modulins promote EV biogenesis by disrupting the cytoplasmic membrane; whereas, peptidoglycan cross-linking and autolysin activity modulate EV production by altering the permeability of the cell wall. We demonstrate that EVs purified from a S. aureus mutant that is genetically engineered to express detoxified cytolysins are immunogenic in mice, elicit cytolysin-neutralizing antibodies, and protect the animals in a lethal sepsis model. Our study reveals mechanisms underlying S. aureus EV production and highlights the usefulness of EVs as a S. aureus vaccine platform.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Infecciones Estafilocócicas / Vacunas Estafilocócicas / Sepsis / Citotoxinas / Anticuerpos Neutralizantes / Vesículas Extracelulares Límite: Animals Idioma: En Revista: Nat Commun Asunto de la revista: BIOLOGIA / CIENCIA Año: 2018 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Infecciones Estafilocócicas / Vacunas Estafilocócicas / Sepsis / Citotoxinas / Anticuerpos Neutralizantes / Vesículas Extracelulares Límite: Animals Idioma: En Revista: Nat Commun Asunto de la revista: BIOLOGIA / CIENCIA Año: 2018 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido