Real-time imaging of specific genomic loci in eukaryotic cells using the ANCHOR DNA labelling system.
Methods
; 142: 16-23, 2018 06 01.
Article
en En
| MEDLINE
| ID: mdl-29660486
ABSTRACT
Spatio-temporal organization of the cell nucleus adapts to and regulates genomic processes. Microscopy approaches that enable direct monitoring of specific chromatin sites in single cells and in real time are needed to better understand the dynamics involved. In this chapter, we describe the principle and development of ANCHOR, a novel tool for DNA labelling in eukaryotic cells. Protocols for use of ANCHOR to visualize a single genomic locus in eukaryotic cells are presented. We describe an approach for live cell imaging of a DNA locus during the entire cell cycle in human breast cancer cells.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Coloración y Etiquetado
/
ADN
/
Imagen Molecular
/
Sitios Genéticos
/
Microscopía Intravital
Límite:
Humans
Idioma:
En
Revista:
Methods
Asunto de la revista:
BIOQUIMICA
Año:
2018
Tipo del documento:
Article
País de afiliación:
Francia