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Isolation and sequence characterization of DNA-A genome of a new begomovirus strain associated with severe leaf curling symptoms of Jatropha curcas L.
Chauhan, Sushma; Rahman, Hifzur; Mastan, Shaik G; Pamidimarri, D V N Sudheer; Reddy, Muppala P.
Afiliación
  • Chauhan S; Department of Chemical and Biochemical Engineering, Dongguk University-Seoul, Seoul 100-715, Republic of Korea.
  • Rahman H; Department of Plant Biotechnology, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu 641003, India.
  • Mastan SG; Department of Biotechnology, Aditya Degree and PG college, Adikavinannaya University, Laxminarayana Nagar, Kakinada 533003, Andhra Pradesh, India.
  • Pamidimarri DVNS; Department of Environmental Engineering, College of Engineering, Ajou University, Suwon, Gyeonggi-do, Republic of Korea. Electronic address: pdvnsudheer@ajou.ac.kr.
  • Reddy MP; Plant Stress Genomics and Technology Center, King Abdullah University of Science and Technology, Thuwal 23955-6900, Saudi Arabia.
Gene ; 664: 37-43, 2018 Jul 20.
Article en En | MEDLINE | ID: mdl-29684487
ABSTRACT
Begomoviruses belong to the family Geminiviridae are associated with several disease symptoms, such as mosaic and leaf curling in Jatropha curcas. The molecular characterization of these viral strains will help in developing management strategies to control the disease. In this study, J. curcas that was infected with begomovirus and showed acute leaf curling symptoms were identified. DNA-A segment from pathogenic viral strain was isolated and sequenced. The sequenced genome was assembled and characterized in detail. The full-length DNA-A sequence was covered by primer walking. The genome sequence showed the general organization of DNA-A from begomovirus by the distribution of ORFs in both viral and anti-viral strands. The genome size ranged from 2844 bp-2852 bp. Three strains with minor nucleotide variations were identified, and a phylogenetic analysis was performed by comparing the DNA-A segments from other reported begomovirus isolates. The maximum sequence similarity was observed with Euphorbia yellow mosaic virus (FN435995). In the phylogenetic tree, no clustering was observed with previously reported begomovirus strains isolated from J. curcas host. The strains isolated in this study belong to new begomoviral strain that elicits symptoms of leaf curling in J. curcas. The results indicate that the probable origin of the strains is from Jatropha mosaic virus infecting J. gassypifolia. The strains isolated in this study are referred as Jatropha curcas leaf curl India virus (JCLCIV) based on the major symptoms exhibited by host J. curcas.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Enfermedades de las Plantas / ADN Viral / Genoma Viral / Jatropha / Begomovirus Tipo de estudio: Diagnostic_studies / Risk_factors_studies Idioma: En Revista: Gene Año: 2018 Tipo del documento: Article

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Enfermedades de las Plantas / ADN Viral / Genoma Viral / Jatropha / Begomovirus Tipo de estudio: Diagnostic_studies / Risk_factors_studies Idioma: En Revista: Gene Año: 2018 Tipo del documento: Article