Simple paired heavy- and light-chain antibody repertoire sequencing using endoplasmic reticulum microsomes.
Genome Med
; 10(1): 34, 2018 04 27.
Article
en En
| MEDLINE
| ID: mdl-29703216
Existing methods for paired antibody heavy- and light-chain repertoire sequencing rely on specialized equipment and are limited by their commercial availability and high costs. Here, we report a novel simple and cost-effective emulsion-based single-cell paired antibody repertoire sequencing method that employs only basic laboratory equipment. We performed a proof-of-concept using mixed mouse hybridoma cells and we also showed that our method can be used for discovery of novel antigen-specific monoclonal antibodies by sequencing human CD19+ B cell IgM and IgG repertoires isolated from peripheral whole blood before and seven days after Td (Tetanus toxoid/Diphtheria toxoid) booster immunization. We anticipate broad applicability of our method for providing insights into adaptive immune responses associated with various diseases, vaccinations, and cancer immunotherapies.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Retículo Endoplásmico
/
Microsomas
/
Anticuerpos
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Genome Med
Año:
2018
Tipo del documento:
Article
País de afiliación:
Alemania
Pais de publicación:
Reino Unido