Paeonol alleviated acute alcohol-induced liver injury via SIRT1/Nrf2/NF-κB signaling pathway.
Environ Toxicol Pharmacol
; 60: 110-117, 2018 Jun.
Article
en En
| MEDLINE
| ID: mdl-29704732
ABSTRACT
In this study, the beneficial effect of paeonol on acute alcohol-induced liver injury and the basic mechanisms were investigated. in vitro, HepG2 cells were treated with paeonol for 24â¯h before it were exposed to alcohol for 24â¯h. in vivo, male C57BL/6 mice were used to establish alcohol-induced liver injury models by oral gavage of alcohol (5â¯g/kg BW). Paeonol pretreatment showed statistically significant reduction in alcohol-induced ROS, MDA, IL-1ß, IL-6, TNF-α, and nitric oxide, while GSH content was retained (Pâ¯<â¯0.05). Furthermore, paeonol treatment resulted in the increase of Nrf2 nuclear translocation, the increase of NQO-1 and HO-1 expression, and the suppression of NF-κB p65 nuclear translocation. However, pretreatment with NAM (inhibitor of SIRT1) not only inhibited the effect of paeonol on reducing nuclear translocation of NF-κBp65, but also inhibited the effect of paeonol on promoting the expression of nuclear Nrf2, NQO1 and HO-1. Besides, paeonol pretreatment at test doses significantly ameliorated alcohol-induced edema, hepatocyte necrosis and hepatic cord irregular. These results demonstrated that paeonol has the high potential for relieving acute alcohol-induced liver injury.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Acetofenonas
/
FN-kappa B
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Alcoholismo
/
Factor 2 Relacionado con NF-E2
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Enfermedad Hepática Inducida por Sustancias y Drogas
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Sirtuina 1
Tipo de estudio:
Etiology_studies
/
Prognostic_studies
Límite:
Animals
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Humans
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Male
Idioma:
En
Revista:
Environ Toxicol Pharmacol
Año:
2018
Tipo del documento:
Article