Photoreceptor Outer Segment Isolation from a Single Canine Retina for RPE Phagocytosis Assay.
Adv Exp Med Biol
; 1074: 593-601, 2018.
Article
en En
| MEDLINE
| ID: mdl-29721992
ABSTRACT
Protocols for photoreceptor outer segment (POS) isolation that can be used in phagocytosis assays of retinal pigment epithelium (RPE) cells have routinely used a large number of cow or pig eyes. However, when working with large animal models (e.g., dog, cats, transgenic pigs) of inherited retinal degenerative diseases, access to retinal tissues may be limited. An optimized protocol is presented in this paper to isolate sufficient POS from a single canine retina for use in RPE phagocytosis assays.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Fagocitosis
/
Retina
/
Fraccionamiento Celular
/
Epitelio Pigmentado de la Retina
/
Cultivo Primario de Células
Límite:
Animals
Idioma:
En
Revista:
Adv Exp Med Biol
Año:
2018
Tipo del documento:
Article
País de afiliación:
Estados Unidos