Photoreceptor Outer Segment Isolation from a Single Canine Retina for RPE Phagocytosis Assay.

Sudharsan, Raghavi; Elliott, Michael H; Dolgova, Natalia; Aguirre, Gustavo D; Beltran, William A

Sudharsan, Raghavi; Elliott, Michael H; Dolgova, Natalia; Aguirre, Gustavo D; Beltran, William A.

Afiliación

Sudharsan R; Division of Experimental Retinal Therapies, Department of Clinical Sciences and Advanced Medicine, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA, USA.
Elliott MH; Department of Ophthalmology, University of Oklahoma Health Science Centre, Oklahoma City, OK, USA.
Dolgova N; Division of Experimental Retinal Therapies, Department of Clinical Sciences and Advanced Medicine, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA, USA.
Aguirre GD; Division of Experimental Retinal Therapies, Department of Clinical Sciences and Advanced Medicine, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA, USA.
Beltran WA; Division of Experimental Retinal Therapies, Department of Clinical Sciences and Advanced Medicine, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA, USA. wbeltran@vet.upenn.edu.

Adv Exp Med Biol ; 1074: 593-601, 2018.

Article en En | MEDLINE | ID: mdl-29721992

ABSTRACT

ABSTRACT

Protocols for photoreceptor outer segment (POS) isolation that can be used in phagocytosis assays of retinal pigment epithelium (RPE) cells have routinely used a large number of cow or pig eyes. However, when working with large animal models (e.g., dog, cats, transgenic pigs) of inherited retinal degenerative diseases, access to retinal tissues may be limited. An optimized protocol is presented in this paper to isolate sufficient POS from a single canine retina for use in RPE phagocytosis assays.

Asunto(s)

Fraccionamiento Celular/métodos; Fagocitosis; Cultivo Primario de Células/métodos; Retina/citología; Epitelio Pigmentado de la Retina/metabolismo; Animales; Células Cultivadas; Perros; Técnica del Anticuerpo Fluorescente Directa; Colorantes Fluorescentes; Rodopsina/análisis; Rodopsina/inmunología; Segmento Externo de la Célula en Bastón; Coloración y Etiquetado/métodos; Proteína de la Zonula Occludens-1/análisis; Proteína de la Zonula Occludens-1/inmunología

Palabras clave

Canine; Inherited retinal degeneration; Phagocytosis; Photoreceptor outer segment isolation; Retinal pigment epithelium

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Fagocitosis / Retina / Fraccionamiento Celular / Epitelio Pigmentado de la Retina / Cultivo Primario de Células Límite: Animals Idioma: En Revista: Adv Exp Med Biol Año: 2018 Tipo del documento: Article País de afiliación: Estados Unidos

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