Characterization of twin-cysteine motif in the V2-loop region of gp120 in primate lentiviruses.
Virology
; 519: 180-189, 2018 06.
Article
en En
| MEDLINE
| ID: mdl-29729526
ABSTRACT
The twin-cysteine motif (TCM) in the V2 loop region of gp120, identified in our previous report on the simian immunodeficiency virus mac239 (SIVmac239), is a conserved evolutionary element in all primate lentiviruses except for HIV-1 which has lost the TCM during cross-species transmission. In this study, we have further explored the TCM in other SIV and HIV-2 strains. Our data shows that strains from different evolutionary lineages have different phenotypes when the twin-cysteines are removed. In the SIVsm/HIV-2 lineage, removal of the twin-cysteines decreases envelope trimer stability, but in the SIVagm lineage, a blockage of gp160 processing is observed. Molecular modeling has confirmed that the twin-cysteines do form a disulfide bond in the gp120 subunit, which interacts with the V1 loop to stabilize the envelope trimer. Therefore, we hypothesize that if the TCM is added back to HIV-1, it will enhance envelope stability for vaccine immunogen design.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Proteína gp120 de Envoltorio del VIH
/
Proteínas del Envoltorio Viral
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VIH-1
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VIH-2
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Virus de la Inmunodeficiencia de los Simios
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Secuencias de Aminoácidos
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Cisteína
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Virology
Año:
2018
Tipo del documento:
Article
País de afiliación:
Estados Unidos