Proteome analysis of the potential serum biomarkers for chronic benzene poisoning.
Environ Toxicol Pharmacol
; 60: 157-164, 2018 Jun.
Article
en En
| MEDLINE
| ID: mdl-29729575
The aim of our study is to seek novel specific biomarkers which could provide clues to the mechanism of chronic benzene poisoning (CBP) and might also be used as specific markers for early detection and diagnosis. In this study, a comparative serological proteome analysis between normal controls and CBP patients at three different levels of poisoning were performed via a 2D-DIGE and MALDI-TOF-MS. As the result a total of 10 proteins were found significantly altered between the normal and the mild, moderate and severe poisoning. The identified differentially expressed proteins were classified according to their molecular functions, biological processes, and protein classes, and three important serum proteins among them, apolipoproteinA-1, alpha-1-antitrypsin and complement C3, were further confirmed by immune turbidimetric analysis for their significant up-regulation in the CBP patients. Our findings suggest that these differential proteins may help elucidate the mechanism of CBP and provide potential biomarkers for diagnosis.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Benceno
/
Proteínas Sanguíneas
/
Regulación hacia Arriba
/
Proteómica
Tipo de estudio:
Prognostic_studies
/
Screening_studies
Límite:
Adult
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Female
/
Humans
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Male
/
Middle aged
Idioma:
En
Revista:
Environ Toxicol Pharmacol
Año:
2018
Tipo del documento:
Article
País de afiliación:
China
Pais de publicación:
Países Bajos