Enhanced production of reactive oxygen species in HeLa cells under concurrent lowdose carboplatin and Photofrin® photodynamic therapy.
Oncol Rep
; 40(1): 339-345, 2018 Jul.
Article
en En
| MEDLINE
| ID: mdl-29749554
Concurrent lowdose carboplatin/Photofrin® photodynamic therapy (ccPDT) has been shown to promote relapsefree complete tumor regression in cervical or endometrial cancer patients as a fertilitypreservation therapy. This study aimed to investigate the molecular mechanism of the enhanced therapeutic efficacy of ccPDT by determining intracellular reactive oxygen species (ROS) and necrotic or apoptotic cell damage in HeLa cells loaded with fluorescent oxidant agents and Photofrin or/and carboplatin under light irradiation. The cytotoxic effects of ccPDT were compared when monitored with a light dose under carboplatin or Photofrin alone. PhotofrinPDT alone did not enhance either hydroxyl radicals (OHâ¢) or superoxide anions (O2â¢-), but a slight enhancement of hydrogen peroxide (H2O2) production was observed. A larger enhancement of ROS production was obtained in a dosedependent manner following ccPDT, especially OH⢠and H2O2, in conjunction with both necrotic and apoptotic cell death, compared with necroticprone PDT alone. The carboplatinmediated Fenton reaction: 2[PtII]2 + H2O2 â [Pt2.25]4 + OH¯+ OH⢠was proposed to explain the dosedependent enhancement of OHâ¢. In conclusion, the therapeutic enhancement of ccPDT in vitro was attributable to the carboplatinmediated synergetic production of OH⪠and apoptotic cellular damage, compared with PhotofrinPDT alone.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Fotoquimioterapia
/
Neoplasias del Cuello Uterino
/
Carboplatino
/
Recurrencia Local de Neoplasia
Límite:
Female
/
Humans
Idioma:
En
Revista:
Oncol Rep
Asunto de la revista:
NEOPLASIAS
Año:
2018
Tipo del documento:
Article
Pais de publicación:
Grecia