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Encapsulation of Exogenous Proteins in Vault Nanoparticles.
Wang, Meng; Abad, Danny; Kickhoefer, Valerie A; Rome, Leonard H; Mahendra, Shaily.
Afiliación
  • Wang M; Department of Civil and Environmental Engineering, University of California, Los Angeles, CA, USA.
  • Abad D; Department of Biological Chemistry, University of California, Los Angeles, CA, USA.
  • Kickhoefer VA; Department of Biological Chemistry, University of California, Los Angeles, CA, USA.
  • Rome LH; Department of Biological Chemistry, University of California, Los Angeles, CA, USA.
  • Mahendra S; Department of Civil and Environmental Engineering, University of California, Los Angeles, CA, USA. mahendra@seas.ucla.edu.
Methods Mol Biol ; 1798: 25-37, 2018.
Article en En | MEDLINE | ID: mdl-29868949
ABSTRACT
Natural vault nanoparticles are ribonucleoprotein particles with a mass of 13 MDa that have been found in a wide variety of eukaryotes. Empty recombinant vaults are assembled from heterologously expressed Major Vault Protein (MVP), forming the barrel-shaped vault shell. These structures are morphologically indistinguishable from natural vault particles. Here, we describe the packaging and purification of exogenous proteins into these recombinant vault particles by mixing with proteins attached to the INT domain that binds to MVP.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Partículas Ribonucleoproteicas en Bóveda / Nanopartículas Límite: Animals Idioma: En Revista: Methods Mol Biol Asunto de la revista: BIOLOGIA MOLECULAR Año: 2018 Tipo del documento: Article País de afiliación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Partículas Ribonucleoproteicas en Bóveda / Nanopartículas Límite: Animals Idioma: En Revista: Methods Mol Biol Asunto de la revista: BIOLOGIA MOLECULAR Año: 2018 Tipo del documento: Article País de afiliación: Estados Unidos