Suction Pipette Technique: An Electrophysiological Tool to Study Olfactory Receptor-Dependent Signal Transduction.
Methods Mol Biol
; 1820: 137-145, 2018.
Article
en En
| MEDLINE
| ID: mdl-29884943
ABSTRACT
The first step to perceive molecules in the air as odors is their detection by the olfactory receptors (ORs) present in the cilia of the olfactory sensory neurons (OSNs) in the nasal cavity. The binding of the odorant molecule to the OR triggers a series of biochemical events that lead to the opening of ion channels, creating at first a generator potential that, if the latter reaches threshold, leads to action potential firing. New insights into olfactory transduction introduced new key players and highlighted the necessity to study OSN physiology in an OR-dependent fashion.The necessity of revisiting transduction mechanisms with consideration of the OR that an OSN expresses requires recording methods of odorant responses at single cell levels. A very effective method to do so is the Suction Pipette Technique, which allows the simultaneous recording of the slow receptor current that originates at the cilia and fast action potentials fired by the cell body. This method can be used in combination with gene targeting and editing techniques to fully address important aspects of the olfactory physiology.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Olfato
/
Potenciales de Acción
/
Transducción de Señal
/
Neuronas Receptoras Olfatorias
Límite:
Animals
Idioma:
En
Revista:
Methods Mol Biol
Asunto de la revista:
BIOLOGIA MOLECULAR
Año:
2018
Tipo del documento:
Article
País de afiliación:
Italia
Pais de publicación:
EEUU
/
ESTADOS UNIDOS
/
ESTADOS UNIDOS DA AMERICA
/
EUA
/
UNITED STATES
/
UNITED STATES OF AMERICA
/
US
/
USA