An improved Ultra-High Performance Liquid chromatography-tandem mass spectrometry method for simultaneous quantitation of cytochrome P450 metabolites of arachidonic acid in human plasma.
J Chromatogr A
; 1563: 144-153, 2018 Aug 17.
Article
en En
| MEDLINE
| ID: mdl-29887336
ABSTRACT
This study aims to develop a straightforward, sensitive UHPLC-MS/MS method to quantify 15 eicosanoids derived from arachidonic acid in human plasma. Tert-Butyl methyl ether was used on the liquid-liquid extraction method and significantly reduced the expense and time. The method showed excellent linearity for all analytes, with regression coefficients higher than 0.99 over a wide range of concentrations from 0.01â¯ngâ¯mL-1 to 100â¯ngâ¯mL-1. The recovery rates were over 65.00%, and the matrix effects ranged from 8.42% to 40.00%. The limits of detection ranged from 6â¯pgâ¯mL-1 to 10â¯pgâ¯mL-1, and all of the limits of quantification were 20 - 33â¯pgâ¯mL-1. For the broad concentration range, the RE% for accuracy and precision were less than⯱â¯15%. Moreover, trans-4-{4-[3-(4-Trifluoromethoxyphenyl)-ureido] cyclohexyloxy} benzoic acid (t-TUCB) pretreatment extended the window of detection for as much as 30â¯days. Eicosanoid signaling is altered in various neurological diseases, including pain, Alzheimer's disease and major depressive disorder. Therefore, this rapid, robust quantitative profiling of 15 eicosanoids in plasma could provide a distinct eicosanoid fingerprint for precision medicine in these patients.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Ácido Araquidónico
/
Sistema Enzimático del Citocromo P-450
Límite:
Humans
Idioma:
En
Revista:
J Chromatogr A
Año:
2018
Tipo del documento:
Article
País de afiliación:
China