[Isolation and purification of restriction endonuclease PmiI from Proteus mirabilis 1667]. / Vydelenie i ochistka restriktsionnoi éndonukleasy PmiI iz shtamma Proteus mirabilis 1667.
Antibiot Med Biotekhnol
; 30(5): 342-4, 1985 May.
Article
en Ru
| MEDLINE
| ID: mdl-2992364
ABSTRACT
A new restriction endonuclease Pmi I was detected in Proteus mirabilis 1667. The enzyme hydrolyzes DNA of the phage lambda into 10 electrophoretically separating fragments with molecular weights of 1.3-7.9 mD. With the use of two-stage chromatography on blue sepharose and phosphocellulose it is possible to obtain restriction endonuclease Pmi I free of the admixtures of ballast proteins, nonspecific nucleases and phosphatases.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Proteus mirabilis
/
Desoxirribonucleasas de Localización Especificada Tipo II
/
Enzimas de Restricción del ADN
Idioma:
Ru
Revista:
Antibiot Med Biotekhnol
Asunto de la revista:
BIOTECNOLOGIA
Año:
1985
Tipo del documento:
Article