Increased antiviral capacity of transgenic silkworm via knockdown of multiple genes on Bombyx mori bidensovirus.
Dev Comp Immunol
; 87: 188-192, 2018 10.
Article
en En
| MEDLINE
| ID: mdl-29944898
ABSTRACT
Bombyx mori bidensovirus (BmBDV) causes fatal ï¬acherie disease leading to severe economic losses in sericultures. The BmDNV-Z genome contains two single-stranded DNA molecules, VD1 and VD2. For generating silkworm lines with antiviral properties, two transgenic RNA interference (RNAi) vectors were constructed. Open reading frames (ORFs) 1-4 of VD1 were knockdown by vector pb-BDV1 while ORF1a, ORF1b, and ORF3 of VD2 were knockdown by vector pb-BDV2. Transgenic silkworm lines BDV1-I and BDV2-I were generated via RNAi microinjection. Mortality rates of BDV1-I and BDV2-I were reduced by 45% and 39%, respectively, and quantitative PCR showed that VD1 and VD2 contents in BDV1-I and BDV2-I were signiï¬cantly lower than in the non-transgenic line. However, economic traits showed no obvious differences. Thus, knockdown of multiple BmDNV-Z genes provides strong resistance to BDV1-I and BDV2-I lines, and these can be used in sericulture without hampering silk production.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Bombyx
/
Interferencia de ARN
/
Genes Virales
/
Virus de Insectos
Límite:
Animals
Idioma:
En
Revista:
Dev Comp Immunol
Año:
2018
Tipo del documento:
Article