In vitro efficacy of silk sericin microparticles and platelet lysate for intervertebral disk regeneration.
Int J Biol Macromol
; 118(Pt A): 792-799, 2018 Oct 15.
Article
en En
| MEDLINE
| ID: mdl-29959012
ABSTRACT
Intervertebral disk degeneration is an oxidative and inflammatory pathological condition that induces viability and functionality reduction of Nucleus Pulposus cells (NPs). Cellular therapies were previously proposed to repair and substitute the herniated disk but low proliferative index and pathological conditions of NPs dramatically reduced the efficacy of this approach. To overcome these problems we proposed, for the first time, a therapeutic system based on the association of silk sericin microparticles and platelet-derived products. Silk sericin (SS) is a bioactive protein with marked antioxidant properties, while platelet lysate (PL) and platelet poor plasma (PPP) represent a source of growth factors able to support cell viability and to promote tissue regeneration. We demonstrated that the mixture PLâ¯+â¯PPP promoted NPs proliferation with a significant reduction of cellular doubling time. SS microparticles, alone or in combination with PPP, presented the higher ROS-scavenging activity while, SS microparticles and PL resulted as the best association able to protect NPs against oxidative stress induce by hydroxide peroxide. Based on these results, the authors are confident that, with the ever increasing need of efficacious tools for regenerative medicine purposes, SS microparticles and PLâ¯+â¯PPP association could represent an effective approach for the development of low impact and non-invasive therapies.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Regeneración
/
Plaquetas
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Sericinas
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Proliferación Celular
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Disco Intervertebral
Límite:
Humans
Idioma:
En
Revista:
Int J Biol Macromol
Año:
2018
Tipo del documento:
Article
País de afiliación:
Italia
Pais de publicación:
HOLANDA
/
HOLLAND
/
NETHERLANDS
/
NL
/
PAISES BAJOS
/
THE NETHERLANDS