MiRNA-34a reversed TGF-ß-induced epithelial-mesenchymal transition via suppression of SMAD4 in NPC cells.
Biomed Pharmacother
; 106: 217-224, 2018 Oct.
Article
en En
| MEDLINE
| ID: mdl-29960168
ABSTRACT
Epithelial-mesenchymal transition (EMT) is considered a prerequisite for tumor invasion and metastasis in many cancers. However, the mechanisms underlying EMT in nasopharyngeal carcinoma (NPC) is largely unknown. In this study, we found that transforming growth factor-ß (TGF-ß), which reportedly promotes EMT in multiple cancers, can trigger EMT and increase the invasive and migratory capacities of NPC cells. Conversely, the downregulation of SMAD4, a vital member of the canonical TGF-ß pathway, reversed the TGF-ß-induced EMT, invasion, and migration. Further experiments revealed that SMAD4 was the target of miRNA-34a, which was downregulated in NPC tissues and suppressed NPC cell metastasis in vivo. miRNA-34a overexpression also antagonized the TGF-ß-induced EMT progression, invasion, and migration through SMAD4 inhibition. However, the restoration of SMAD4 expression rescued the inhibitory effects of miRNA-34a on tumorigenesis. All these results confirmed that miRNA-34a suppressed the TGF-ß-induced EMT, invasion, and migration of NPC cells by directly targeting SMAD4, which indicated the potential of miR-34a as a therapeutic target against NPC.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Carcinoma
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Neoplasias Nasofaríngeas
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Factor de Crecimiento Transformador beta
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MicroARNs
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Proteína Smad4
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Transición Epitelial-Mesenquimal
Límite:
Animals
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Humans
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Male
Idioma:
En
Revista:
Biomed Pharmacother
Año:
2018
Tipo del documento:
Article
País de afiliación:
China