Development of a Recombinant Protein-based ELISA for Detection of Antibodies Against Bovine Foamy Virus.
J Vet Res
; 61(3): 247-252, 2017 Sep.
Article
en En
| MEDLINE
| ID: mdl-29978080
ABSTRACT
INTRODUCTION:
Infections with bovine foamy virus (BFV) were found in many countries but there is a lack of large-scale surveys on the prevalence of BFV among dairy cattle. The aim of this study was to develop and validate the recombinant Gag protein-based ELISA and to estimate the prevalence of antibodies against BFV. MATERIAL ANDMETHODS:
Gag coding region from BFV was cloned into expression vector pT7Arg-STOP, which expressed a high level of recombinant Gag protein from E.coli. The ELISA was standardised, and the cut-off value and sensitivity and specificity of the test were calculated using a receiver operating characteristic and Bayesian estimation.RESULTS:
A total of 3,051 serum samples were tested by ELISA and 939 (30.8%) sera were recognised as positive. When Bayesian approach was used, the overall true BFV prevalence was 29.7% (95% CI 25.9-33.4%).CONCLUSION:
Expressed Gag protein of BFV has been used successfully as an antigen for ELISA. Eventually, this study provides basic information about the epidemiological status of infection with BFV in dairy cattle in Poland, which can be used for further studies on dissemination and transmission of BFV infection.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Tipo de estudio:
Diagnostic_studies
/
Risk_factors_studies
Idioma:
En
Revista:
J Vet Res
Año:
2017
Tipo del documento:
Article
País de afiliación:
Polonia