Upregulation of Connexin-43 is Critical for Irradiation-induced Neuroinflammation.
CNS Neurol Disord Drug Targets
; 17(7): 539-546, 2018.
Article
en En
| MEDLINE
| ID: mdl-29984671
ABSTRACT
BACKGROUND:
Radiation therapy is widely used for the treatment of pituitary adenomas. Unfortunately, it might raise the risk of ischemic stroke, with neuroinflammation being a major pathological process. Astrocytes are the most abundant cell type in the central nervous system and have been reported for playing important roles in ischemic stroke.OBJECTIVE:
Here we studied how γ-radiation would introduce astrocytes into a detrimental state for neuroinflammation and provide new theory evidence and target for the clinical management of inflammation- related neural damage after radiation-induced ischemic stroke.METHOD:
HA-1800 cells were treated with γ-radiation and then the protein and mRNA levels of Connexin (Cx)-43 were evaluated by western and q-PCR. The culture supernatant was collected and the concentrations of the inflammatory factors were determined by ELISA. MiRNA complementary to Cx-43 was designed through the online tools.RESULTS:
Cx-43 is upregulated in the treatment of γ-radiation in astrocytes and γ-radiation introduced the detrimental function of astrocytes cell viability was reduced while the apoptotic cells were increased. Inflammatory factors like tumor necrosis factor alpha, interferon gamma, interleukin-6, interleukin 1-beta were dramatically up-regulated by the irradiation. MiR-374a rescued irradiation induced Cx-43 up-regulation of astrocytes and eliminated detrimental function triggered by γ-radiation.CONCLUSION:
Cx-43 expression level may play an important role in the inflammation-related neural damage after irradiation-induced ischemic stroke.Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Regulación hacia Arriba
/
Neuroglía
/
Citocinas
/
Apoptosis
/
Conexina 43
/
Rayos gamma
Límite:
Humans
Idioma:
En
Revista:
CNS Neurol Disord Drug Targets
Asunto de la revista:
NEUROLOGIA
/
TERAPIA POR MEDICAMENTOS
Año:
2018
Tipo del documento:
Article
País de afiliación:
China