Expression of transmembrane protein 41A is associated with metastasis via the modulation of Ecadherin in radically resected gastric cancer.
Mol Med Rep
; 18(3): 2963-2972, 2018 Sep.
Article
en En
| MEDLINE
| ID: mdl-30015937
ABSTRACT
Gastric cancer (GC) is one of the most commonly occurring malignancies worldwide, and metastasis is one of the key processes affecting the prognosis of GC. TMEM41A, which belongs to a group of transmembrane proteins that participate in signaling pathways and tumor development, is a 264amino acid protein encoded by a gene mapped to human chromosome The exact role of TMEM41A in GC has not been determined to date. In the present study, the expression of TMEM41A in 147 cases of GC was analyzed with immunohistohemistry and the prognoses of these patients were analyzed. It was revealed that TMEM41A was highly expressed in GC tissues, and may be associated with the progression of GC and poor prognosis. The expression of TMEM41A was observed to be correlated with lymph node metastasis, distant metastasis and advanced tumor, node and metastasis stages. Knockdown of TMEM41A in vitro and in vivo decreased the GC cell migration ability by regulating epithelialtomesenchymal transition and cell autophagy, via the upregulation of Ecadherin and downregulating Ncadherin expression in GC cells by reverse transcriptionquantitative polymerase chain reaction (PCR), semiPCR and western blotting. Furthermore, TMEM41A upregulation was associated with the upregulation of p62 and altered the conversion of light chain (LC)31 into LC32 by western blotting. Knockdown of TMEM41A was also observed to affect tumor metastasis in nude mice. Therefore, TMEM41A may be considered as a novel therapeutic target for the treatment of GCassociated metastasis.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Neoplasias Gástricas
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Cadherinas
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Regulación Neoplásica de la Expresión Génica
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Proteínas de la Membrana
Tipo de estudio:
Risk_factors_studies
Límite:
Adult
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Aged
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Aged80
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Animals
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Female
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Humans
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Male
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Middle aged
Idioma:
En
Revista:
Mol Med Rep
Año:
2018
Tipo del documento:
Article
Pais de publicación:
GR
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GRECIA
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GREECE
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GRÉCIA