Development and validation of a simple chromatographic method for simultaneous determination of clindamycin phosphate and rifampicin in skin permeation studies.
J Pharm Biomed Anal
; 159: 331-340, 2018 Sep 10.
Article
en En
| MEDLINE
| ID: mdl-30025298
ABSTRACT
Rifampicin (RIF) and clindamycin phosphate (CDM) are the main drugs currently used in combination to treat severe infectious diseases in hair follicles. This work describes a simple, rapid and sensitive method for simultaneous analysis of RIF and CDM in the different skin layers using high performance liquid chromatography (HPLC). The efficient chromatographic separation of CDM and RIF was succeeded using a C18 column (150â¯mmâ¯xâ¯4.6â¯mm, 5⯵m) with gradient elution using a mobile phase composed of 0.01â¯M phosphoric acid and methanol at a flow rate of 1â¯mL min-1. Determinations were performed using UV-vis detector at 200â¯nm and 238â¯nm for CDM and RIF, respectively. The method was precise, accurate and linear (r2 > 0.999) with regression curve in the concentration range from 0.5 to 20.0⯵gâ¯mL-1 and recovery rates from the skin layers higher than 85%. The retention times for CDM and RIF were approximately 7.4 and 12.2â¯min, respectively. The presence of skin components did not interfere with the analysis. The validated method was therefore appropriate for quantification of both CDM and RIF and thus may be feasible to be used in skin permeation studies.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Rifampin
/
Absorción Cutánea
/
Clindamicina
/
Técnicas de Química Analítica
Límite:
Animals
Idioma:
En
Revista:
J Pharm Biomed Anal
Año:
2018
Tipo del documento:
Article
País de afiliación:
Brasil