Endogenous "ouabain-like" activity in bovine plasma.
Biochem Biophys Res Commun
; 135(1): 1-8, 1986 Feb 26.
Article
en En
| MEDLINE
| ID: mdl-3006675
ABSTRACT
An endogenous inhibitor of the sodium pump has been detected and concentrated 1000-fold from bovine plasma. The steps of purification included deproteinization and extraction with methanol, removal of lipids by coextractions with a lipophilic solvent, desalting and further concentration by adsorption on C18-SepPack cartridges and HPLC fractionation on a weak anionic exchange column. The material isolated displaces 3H-ouabain from brain synaptosomes, inhibits red cell membrane Na,K-ATPase without inhibiting Mg-ATPase or Ca,Mg-ATPase. Deproteinization of plasma by boiling may lead to appearance of non-specific inhibitors. The procedures developed should now permit isolation of sufficient amount of material for further purification and structural characterization.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Ouabaína
/
ATPasa Intercambiadora de Sodio-Potasio
/
Inhibidores Enzimáticos
Límite:
Animals
Idioma:
En
Revista:
Biochem Biophys Res Commun
Año:
1986
Tipo del documento:
Article