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Amount of daily exercise is an essential stimulation to alter the epigenome of skeletal muscle in rats.
Ohsawa, Ikumi; Konno, Ryotaro; Masuzawa, Ryo; Kawano, Fuminori.
Afiliación
  • Ohsawa I; Department of Sports and Health Science, Faculty of Human Health and Science, Matsumoto University, Matsumoto City, Japan.
  • Konno R; Department of Sports and Health Science, Faculty of Human Health and Science, Matsumoto University, Matsumoto City, Japan.
  • Masuzawa R; Graduate School of Health Sciences, Matsumoto University, Matsumoto City, Japan.
  • Kawano F; Department of Sports and Health Science, Faculty of Human Health and Science, Matsumoto University, Matsumoto City, Japan.
J Appl Physiol (1985) ; 125(4): 1097-1104, 2018 10 01.
Article en En | MEDLINE | ID: mdl-30070609
Long-term running training causes epigenetic changes in the skeletal muscles. Here we tested the effects of the total amount or duration of running training on the distribution of histones in the rat plantaris muscle. Post-weaned young rats were assigned to 3 different training groups: Run-1, 30 min/day running exercise for 8 wk using an animal treadmill at 24 m/min; Run-2, 15 min/day for 8 wk; and Run-3, 60 min/day for 4 wk. Citrate synthase activity was not significantly changed by running training, although the slight increase was observed in Run-3. Genes that were previously defined as showing the typical responses to running training were targeted to measure the distribution of histones using chromatin immunoprecipitation. The distribution of acetylated histone 3 was elevated in Run-2 and Run-3, but not in Run-1. Incorporation of H3.3 into the nucleosome was stimulated in Run-1, whereas H3.3 distribution was unchanged in Run-2 or downregulated in Run-3. Significant downregulation of H3.3 expression was also detected in Run-3. We further checked the responses of the target genes during acute running. Target genes were transcriptionally activated and histone acetylation was stimulated at the loci in response to acute running. These results suggested that the exchange of the histone component to H3.3 was stimulated by running training, inhibiting the accumulation of acetylated histones in Run-1. Additionally, it was further suggested that the enhanced daily amount of running caused changes in the H3.3 expression, affecting the rate of the histone exchange in Run-3. NEW & NOTEWORTHY Chromatin remodeling in the skeletal muscle is a potent mechanism preventing disuse atrophy in later life that can be acquired via long-term exercise training. Here we demonstrated in rats that daily exercise amount is a key factor in the development of epigenetic changes in the skeletal muscle. To acquire a health benefit, our research suggests the importance of considering the time endurance for daily exercise bouts.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Condicionamiento Físico Animal / Histonas / Músculo Esquelético / Epigénesis Genética Límite: Animals Idioma: En Revista: J Appl Physiol (1985) Asunto de la revista: FISIOLOGIA Año: 2018 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Condicionamiento Físico Animal / Histonas / Músculo Esquelético / Epigénesis Genética Límite: Animals Idioma: En Revista: J Appl Physiol (1985) Asunto de la revista: FISIOLOGIA Año: 2018 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Estados Unidos