Genome-wide evidences of bisphenol a toxicity using Schizosaccharomyces pombe.
Arch Pharm Res
; 41(8): 830-837, 2018 Aug.
Article
en En
| MEDLINE
| ID: mdl-30099677
ABSTRACT
To clarify reliable toxic mechanisms of bisphenol A (BPA), an endocrine disrupting chemical, we approached an alternative animal and whole genome analyses with the yeast knockout library (YKO) of Schizosaccharomyces pombe. As results, the 50% growth inhibition concentrations (GI50) of BPA was approximately 600 µM and the YKO-three step screening revealed the top 10 target candidate genes including dbp2, utp18, srs1, tif224, use1, qcr1, etc. The screening results were confirmed in human embryonic stem cell (hES)-derived hepatic cells and HepG2 human liver cancer cells. We found BPA down-regulated UQCRC, the human orthlog of S. pombe- qcr1, as a part of the mitochondrial respiratory chain, in HepG2 cells and hESs during cell differentiation into hepatic cells. Therefore, BPA may induce mitochondrial dysfunction and disruption of differentiation by suppressing UQCRC1.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Fenoles
/
Schizosaccharomyces
/
Compuestos de Bencidrilo
Límite:
Humans
Idioma:
En
Revista:
Arch Pharm Res
Año:
2018
Tipo del documento:
Article