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Resonance assignments for the apo-form of the cellulose-active lytic polysaccharide monooxygenase TaLPMO9A.
Kitaoku, Yoshihito; Courtade, Gaston; Petrovic, Dejan M; Fukamizo, Tamo; Eijsink, Vincent G H; Aachmann, Finn L.
Afiliación
  • Kitaoku Y; Biochemistry-Electrochemistry Research Unit, Institute of Science, Suranaree University of Technology, Nakhon Ratchasima, 30000, Thailand.
  • Courtade G; NOBIPOL, Department of Biotechnology and Food Science, NTNU Norwegian University of Science and Technology, Sem Sælands vei 6/8, 7491, Trondheim, Norway.
  • Petrovic DM; Faculty of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences, 1432, Ås, Norway.
  • Fukamizo T; Biochemistry-Electrochemistry Research Unit, Institute of Science, Suranaree University of Technology, Nakhon Ratchasima, 30000, Thailand.
  • Eijsink VGH; Faculty of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences, 1432, Ås, Norway.
  • Aachmann FL; NOBIPOL, Department of Biotechnology and Food Science, NTNU Norwegian University of Science and Technology, Sem Sælands vei 6/8, 7491, Trondheim, Norway. finn.l.aachmann@ntnu.no.
Biomol NMR Assign ; 12(2): 357-361, 2018 10.
Article en En | MEDLINE | ID: mdl-30117034
ABSTRACT
The apo-form of the 24.4 kDa AA9 family lytic polysaccharide monooxygenase TaLPMO9A from Thermoascus aurantiacus has been isotopically labeled and recombinantly expressed in Pichia pastoris. In this paper, we report the 1H, 13C, and 15N chemical shift assignments, as well as an analysis of the secondary structure of the protein based on the secondary chemical shifts.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Apoenzimas / Celulosa / Resonancia Magnética Nuclear Biomolecular / Oxigenasas de Función Mixta Idioma: En Revista: Biomol NMR Assign Asunto de la revista: BIOLOGIA MOLECULAR / MEDICINA NUCLEAR Año: 2018 Tipo del documento: Article País de afiliación: Tailandia
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Apoenzimas / Celulosa / Resonancia Magnética Nuclear Biomolecular / Oxigenasas de Función Mixta Idioma: En Revista: Biomol NMR Assign Asunto de la revista: BIOLOGIA MOLECULAR / MEDICINA NUCLEAR Año: 2018 Tipo del documento: Article País de afiliación: Tailandia